pGTM plasmid细胞膜表达载体MC4100

质粒/菌株

质粒改造
质粒抗性改造组成型/诱导型荧光质粒质粒复制起点更换质粒启动子替换合成生物学质粒构建
现货质粒菌株
大肠杆菌载体 E.coli Vector 大肠杆菌宿主菌株 E.coli 细菌广宿主载体 bacateria broad range host vector 链霉菌载体及菌株 Streptomyces 芽孢杆菌载体 Bacillus vector 芽孢杆菌宿主菌株乳酸菌载体 lactic acid bacteria vector 乳酸菌宿主菌株 lactic acid bacteria strain 细菌基因敲除载体毕赤酵母载体毕赤酵母宿主菌株酿酒酵母载体酿酒酵母宿主菌株丝状真菌载体 mold/fungi vector 乳酸克鲁维酵母载体酵母真菌基因敲除基因编辑载体植物细胞载体 plant cell vector 农杆菌菌株Agrobacterium tumefaciens strain 植物细胞基因敲除载体 plant cell 哺乳动物细胞载体哺乳动物细胞荧光载体荧光素酶报告基因载体哺乳动物细胞基因敲除基因编辑载体杂交系统慢病毒载体腺病毒载体逆转录病毒载体杆状病毒表达载体基因干扰 RNAi载体基因/cDNA/ORF 转座子质粒系统 transposon 金黄色葡萄球菌载体 staphylococcus aureus 假单胞菌载体噬菌体 phage 不动杆菌载体双岐杆菌载体藻类表达载体链球菌载体厌氧菌载体基因治疗载体大肠杆菌基因突变体菌株细菌荧光质粒白色念珠菌载体体外转录载体谷氨酸棒杆菌载体酿酒酵母基因突变体菌株线虫载体斑马鱼载体 Zebra fish 果蝇，昆虫载体Drosophila 鱼类细胞载体 fish cell 分支杆菌载体克雷伯菌枯草芽孢杆菌基因缺失突变株基因ORF
工程菌菌株
质粒菌株资料

产品编号	产品名称
	pGTM plasmid细胞膜表达载体MC4100

产品介绍

The pcDNA3 mammalian expressionvector uses the cytomegalovirus (CMV)promoter to express proteins cloned into anadjacent polylinker. We have modified this vector to create three different targetingconstructs: Go to Plasma Membrane(pGTM), Go to Nucleus (pGTN) and Go toNucleus, Activate Transcription (pGTNAT).pGTM expresses a protein as a fusion toamyristic acid attachment signal, which tar-gets proteins to the cell membranes. pGTNexpresses an inserted protein as a fusion toa nuclear localization sequence (NLS), tar-geting it to the nucleus. pGTNAT incorpo-rates an NLS (directing proteins to the nu-clear compartment), an acid blob (atranscriptional activation domain) andahemagglutinin epitope tag, creating a 107-amino acid fusion domain. We then clonedgreen fluorescent protein (GFP) into thethree novel vectors and pcDNA3, and wetransfected HeLa cells to test the new tar-geting constructs. Immunofluorescenceanalysis showed that the GFP protein lo-calizes to the nucleus when over-expressedin pGTN and pGTNAT, localizes to theplasma membrane and perinuclear mem-brane when in pGTM and is ubiquitousthrough the cell in pcDNA3. We anticipatethat these new vectors will prove very use-ful in future expression studies to examinethe function of particular proteins whenthey are localized to specific cellular com-partments.