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金黄色葡萄球菌载体 staphylococcus aureus
金黄色葡萄球菌荧光质粒pLOW-gfp
发布时间:2022-08-12 17:10:50 | 浏览次数:

IPTG诱导的,金黄色葡萄球菌绿色荧光质粒

pLOW-GFP金黄色葡萄球菌荧光表达载体

Plasmid pLOW, designed for regulated expression of S. aureus proteins. The MCS is shown in full, with the reading frame indicated by the triplet arrangement of the nucleotide sequence. Only unique restriction sites are shown. Genes: ApR, E. coli blaM gene for b-lactamase; ColEI-HC, high-copy-number E. coli plasmid origin of replication; EmR, S. aureus gene originating from plasmid pE194 and encoding Em-resistance gene ermC; pSK41 ori, lowcopy-number S. aureus origin of replication; LacI, lac repressor protein constitutively expressed from the penicillinase promoter Ppcn. (b) Plasmid pLOW-GFP, designed for producing protein– GFP fusions in S. aureus. GFP, gfpmut-1 gene encoding GFP. 

 
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