产品介绍

MUCICAT system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.

• pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right end; LE, left end)
• pTnsABC expresses the transposases TnsA, TnsB, and TnsC
• pQCascade expresses the transposase TniQ, the Cas proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple different sites in E. coli.
• pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed by sacB counterselection. 

The MUCICAT toolkit includes versions of plasmids with different induction systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters) in metabolic engineering.

参考文献：

Multicopy chromosomal integration using CRISPR-associated transposases.
Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S
ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073.  PubMed  Article

基本信息