产品介绍

FEATURES             Location/Qualifiers  source          1..4740
                     /organism="Expression vector pEMCJH04"
                     /mol_type="other DNA"
                     /db_xref="taxon:260048"
                     /clone="3.9"
                     /lab_host="Moraxella catarrhalis"
                     /note="derived from plasmid pEMCJH03 containing random
                     insert of transposon EZ::TN<Kan2>"  oriT 92..204
                     /note="putative"  CDS 230..1147
                     /codon_start=1
                     /transl_table=11 /product="putative replicase"
                     /protein_id="AAR88169.1"
                     /translation="MQNINFSYDLALIQAQILEKLPQKPYCTDDLGYLVVRPKEIAIQ
                     KRYIQHNPPCFTSFLVFDLDTELGAVAWHDADLPLPTWTTQNPDNGHAHIAYALKTPV
                     VTTEQGSNRAIDYLAKIQAGMARKLGADTGYSGLITKNPLHNHWRTTVWNTKPYELGE
                     LADRVELAPLTPKERELGLGRNCTLFDTVRKWAYIAIREHRGGRYDDWYARVLATVQA
                     ENMAFLEPLPYSEIKATAKSIAVYCWKNDGHCYNEFIYRQRLKGAIGGKKSKRPPMVD
                     SERTLQPWIELGISRRTYYRDLNDNHSQK"  rep_origin 1053..1414
                     /note="putative oriV"  gene 1488..>1746
                     /gene="putative mobilization gene C"
                     /note="disrupted by transposon insertion"  mobile_element 1747..2967
                     /mobile_element_type="transposon:EZ::TN<Kan2>"  CDS 1884..2699
                     /function="kanamycin resistance"
                     /codon_start=1
                     /transl_table=11 /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="AAR88170.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"  gene <2976..3058
                     /gene="putative mobilization gene C"
                     /note="disrupted by transposon insertion"  CDS 3281..4543
                     /codon_start=1
                     /transl_table=11 /product="putative mobilization protein A"
                     /protein_id="AAR88171.1"
                     /translation="MASFERTLMAGLNQDRYNILWVEHTDKDRLELNFLIPKVDLGTG
                     KAMNPYFDKTDRGLVDVWKQVINYDYGLHDPDDPKNRQTLVTVKDLPKSKQEFKQALT
                     AVLEQKILADEIKDHADIIKELENMGLEIARTTPTAISIKDPDGGRNIRLKGEIYEQT
                     FTANQATERESQRASESYRNELEQRISRVRDELTSRIEAKSAFNATRYKTIPSREQSP
                     NEQAHGIQDPSRGSNGDFVINPDSIRGVQSVLGQENSHTARAIRRYTTGDRQQPPTSQ
                     STNESTGNGTGRQDLHRQQDEQSQNMAKQRQTTNHGATLNVKAIPERVRAIATRARTL
                     LVIARDGKSDAQATDRAITATNSGLRDRKQQANDRKQRTVEIIGVAKNAGAGIDQHHA
                     EQVRLQQQQARQARQQTKDEPKKLGLDR" ORIGIN  1 atacgtcaaa aagcaccgcc aagcggaact gaagttttga cggattgggc gagccatgat 61 aaattcaagg cgtaaaaaaa gcaggttggc agacctgcga tttttacacg tttatttttg 121 tggcttggta actatagcaa acagtcaaaa cacaccaaaa ataaacccta actaagtata 181 ttttacctgt gttttgactg tttgcaacca ttttttggac tttttgcaaa tgcaaaacat 241 aaatttttca tacgacttgg cgttaatcca agctcaaatc ttagaaaaac tgccccaaaa 301 gccttattgt acggacgatt tgggctatct agtcgttcgc cctaaagaaa ttgcaattca 361 aaaacgctac atacagcata atccgccctg ttttacttct ttccttgtct ttgacctaga 421 tacagagcta ggggcggtag cgtggcatga tgctgattta ccgctaccaa cttggacgac 481 ccaaaaccct gataacggac acgcacacat agcgtacgca ctcaaaacgc ctgtagtaac 541 cacagaacaa ggcagtaacc gagctattga ctacttagcc aagatacaag caggaatggc 601 gagaaaacta ggggcggata caggctatag cggtttaatt accaaaaatc cactacataa 661 ccattggcgt acaacggttt ggaataccaa gccgtacgaa ttaggcgagc ttgccgaccg 721 tgtagagctt gcaccgctca caccaaaaga gcgagagtta gggctagggc gtaactgcac 781 cctatttgat acggtgcgaa aatgggcgta tatcgctatc agagagcata ggggcggtag 841 gtatgatgat tggtacgcta gagtgctagc gactgtccaa gctgaaaata tggcgttttt 901 agagccatta ccatacagcg aaattaaagc caccgccaaa tctattgccg tgtactgttg 961 gaaaaatgac ggacattgct acaatgagtt tatctaccgc caacgcctta agggtgcaat 1021 aggcggtaag aagtccaaac gcccaccaat ggtggacagc gaacgcacac tacaaccttg 1081 gatagagtta ggaattagta gacggacata ctacagggat ttaaatgata atcattctca 1141 aaaatgacac cagataagcc taaatcagat aacagcccca aaagcgagct tttggggtgc 1201 cttttagacg gtgctaggtt tttgacagca gataagccta aatcagataa cagccgaatc 1261 gataagcctt agttggttaa gggggcagga aattcatatt gaacaaatgt ttagttaagt 1321 gtagaataat catacatcct tattaagggc aagcatactc aagccccaca aagtgtgctt 1381 gaaatccttg taaggggaaa tcccccttaa ccccctgcac aatcttcgtt gcactgcgat 1441 tgtgttgggg caagccccaa accccagtta gaaagacaaa gaaaaaaatg agcagagaac 1501 ttagaacaaa gtcaatcaag gttaggctga caccgtccga gttggagcaa ctcaagcaac 1561 ggcaggtagg cgataatcta gctaaatggg tgcgtgatgt ttgcttggct aatccaattc 1621 aagagcctaa gaaattaaaa cgacagtcaa aggttgtaag ggttgctggc cctgatttat 1681 tgagagagct tgcaaagatt gggggaaacc ttaatcagat tgcacgccat gctaacacct 1741 atgatgctgt ctcttataca catctcaacc atcatcgatg aattgtgtct caaaatctct 1801 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 1861 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc 1921 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 1981 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt 2041 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 2101 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 2161 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat 2221 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 2281 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 2341 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 2401 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag 2461 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 2521 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 2581 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 2641 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 2701 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 2761 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga 2821 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa 2881 caaagctctc atcaaccgtg gcggggatcc tctagagtcg acctgcaggc atgcaagctt 2941 cagggttgag atgtgtataa gagacagcct atgatgaccg catagagaaa ttaagagcgt 3001 ttagagcatt ggcgagcatt gaaagtcagc ttgatgaact tttggagcgt tacaagtgat 3061 tgtgaagttt ttcaagaaca agggtgcagg cagtgctaca gcgagcgtaa attacttgtt 3121 aggcaaggat agggataggc aaggggctag ggtgctacat ggcaatgcag agcttacagc 3181 acagcttgca gagagcctag agtttaaaca caaatatacc gttggttgtc tatcatttgc 3241 tgagcaggac aagctagacg agaacgctaa gcgtgaaatc atggcaagtt ttgagcgtac 3301 gctcatggca gggttaaacc aagaccgcta caacattttg tgggttgaac acaccgataa 3361 agaccgccta gagcttaatt ttttaatccc aaaggttgat ttgggcacag gcaaggcgat 3421 gaacccttac tttgacaaga ccgatagggg gcttgtagat gtgtggaaac aggtaattaa 3481 ctatgactat gggctacacg acccagacga ccccaagaac cgccaaacgc ttgtaaccgt 3541 caaagacctg ccaaagagta agcaagaatt taagcaagcc ctaacagcag ttttagagca 3601 gaaaatttta gccgatgaaa tcaaagacca tgcagacatc atcaaagagc tagaaaacat 3661 ggggctagaa atcgcacgga cgacaccaac cgcaatcagt atcaaagacc ctgatggggg 3721 gcgaaacata cgactaaagg gcgaaatcta tgagcagact ttcacagcta accaagcaac 3781 tgaacgagaa agccaacgag caagcgaaag ctacagaaat gagcttgaac agcgtatttc 3841 aagagttcga gacgaactta caagccgaat tgaagcaaag tcagcattca atgctacacg 3901 ctataaaaca atcccaagcc gagagcaaag cccaaatgaa caagctcatg gcatacaaga 3961 ccctagcagg ggtagcaatg gggattttgt tatcaatcct gatagcatta ggggcgttca 4021 gtctgtactt gggcaagaaa atagccacac agcaagggca attagacgat atacaacagg 4081 cgatagacaa cagcccccta caagccaaag cactaacgaa agtacaggta acggaacagg 4141 acggcaagat ttacatcgtc agcaagacga gcaaagccaa aacatggcaa aacaaagaca 4201 aacaacaaat catggagcta ccctaaatgt taaagccatt cctgaacgag tacgagcaat 4261 cgctacaaga gctagaacgc tacttgtcat cgctagagac ggaaaatcgg acgctcaagc 4321 gacagataga gccattacag cgacaaatag cggacttaga gaccgaaaac agcaagctaa 4381 cgaccgaaaa caacgaacag ttgaaattat tggagttgct aaaaatgcag gtgcaggaat 4441 tgaccaacat catgcagaac aggtaaggct acaacagcaa caggcacgcc aagcacgaca 4501 acagaccaaa gatgagccga aaaaattagg cttagaccgt taaaaagttc ttgttttgtc 4561 tttttaattg caatacaatg tattgcgaaa attatacatt aaggtgtaat cgatgagcga 4621 aaaagcccca aaaacagcta taacatttcg cattgacgaa agcctagcta agcaatttaa 4681 ggaaattgca gaagccaaca accgcactca gtcgctacta ctgcgtgatt ttattattga

基本信息