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大肠杆菌宿主菌株 E.coli

LOBSTR E. coli Expression Strain 镍株纯化低背景杂蛋白,类似NEB Nico21 菌株

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LOBSTR E. coli Expression Strain 镍株纯化低背景杂蛋白,类似NEB Nico21 菌株

产品介绍

大肠杆菌 BL21 (DE3)衍生菌株,可以有效降低His标签纯化过程中的杂蛋白吸附,提供更纯的蛋白。

His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL21(DE3) strain and carries genomically modified copies of arnA and slyD, whose protein products exhibit reduced affinities to Ni and Co resins, resulting in a much higher purity of the target protein. The use of LOBSTR enables the pursuit of challenging low expressing protein targets by reducing background contamination with no additional purification steps, materials, or costs, and thus pushes the limits of standard His-tag purifications.

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