产品介绍

Name: pNF2

DSM No.: 4862 dsm-4862 dsm4862 dsm 4862 pNF2

Other collection no.or WDCM no.: ATCC 37187

History: <- E.C. Friedberg

Mol. weight: 10.3 kb

Marker: Apr and Kmr in E. coli, G418r and Ura+ in yeast

Remarks: Cloning vector with 2μ plasmid replicon for Saccharomyces cerevisiae and Escherichia coli. pNF2 is a derivative of the multicopy yeast cloning vector YEp24 and contains a single BamHI cloning site flanked immediately on each side by SalI sites. DNA fragments cloned into the BamHI site that do not regenerate this restriction site can be excised by digestion with SalI enzyme. The Kmr gene derived from Tn903 confers resistance in yeast to the antibiotic G418.

Distributed in: Escherichia coli K12 HB101

Cultivation conditions: Medium 381, 37°C

基本信息