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DSM 4514 pUB110

产品编号 产品名称
DSM 4514 pUB110

产品介绍

Name: pUB110

DSM No.: 4514 dsm-4514 dsm4514 dsm 4514 pUB110

Other collection no.or WDCM no.: ATCC 37015, NCIB 11624

History: <- ATCC <- D. Dubnau

Mol. weight: 4.5 kb

Marker: Kmr, Neor

Remarks: Natural isolate. Originally discovered in Staphylococcus aureus. General purpose plasmid vector for cloning in Bacillus. Sequence known. Copy number in B. subtilis about 50. The pUB110 replicon is the basis for the construction of chimeric plasmid vectors. The neomycin resistance is expressed in E. coli. Kmr and Neor may be insertionally inactivated within the unique BglII or BanI sites. pUB110 can replicate in other Bacillus species. Transfer of the vector either via transformation or transduction, using transducing phages PBS1, AR9 or SP10.

Distributed in: Bacillus subtilis subsp. subtilis BD170 (derivative of strain 168)

Cultivation conditions: Medium 381, 30°C

基本信息

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