产品介绍

Species: Escherichia phage phiX174 (Sinsheimervirus phiX174)

Taxonomy: Order: Petitvirales

Family: Microviridae

Subfamily: Bullavirinae

Genus: Sinsheimervirus

Isolate: Escherichia phage phiX174

DSM No.: 4497 dsm-4497 dsm4497 dsm 4497 Sinsheimervirus phiX174

History: <- ATCC <- R.L. Sinsheimer

Geographic origin: country of origin unknown

Date of sampling: before 18.02.1993

Nagoya Protocol Restrictions: There are NO known Nagoya protocol restrictions for this strain.

Genomics: ssDNA

Morphology: icosahedral capsid with 12 spikes, no tail, capsid size 25 nm

Plaques description: large plaques with a clear centre and turbid halo

Image: ###IMAGE_TPL###

Single plaques on agar plate.

Creator: B. Henze, Leibniz Institute DSMZ.

© © Leibniz-Institut DSMZ

Further information: Prior to infection, the phage undergoes a conformational change and forms an eclipsed particle. The phage does not produce high titers above 1E+8 pfu/mL. We recommend to perform lysis on plates, not in liquid culture

Literature: 6286, 15856, 23821

Risk group: 1

Phage propagation

Host bacterium: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

DSM-13127

We strongly recommend to use only DSM-13127 for propagation.

Cultivation conditions: Medium 381 , 37°C

基本信息