大肠杆菌BW25141(λDE3)

质粒/菌株

质粒改造
质粒抗性改造组成型/诱导型荧光质粒质粒复制起点更换质粒启动子替换合成生物学质粒构建
现货质粒菌株
大肠杆菌载体 E.coli Vector 大肠杆菌宿主菌株 E.coli 细菌广宿主载体 bacateria broad range host vector 链霉菌载体及菌株 Streptomyces 芽孢杆菌载体 Bacillus vector 芽孢杆菌宿主菌株乳酸菌载体 lactic acid bacteria vector 乳酸菌宿主菌株 lactic acid bacteria strain 细菌基因敲除载体毕赤酵母载体毕赤酵母宿主菌株酿酒酵母载体酿酒酵母宿主菌株丝状真菌载体 mold/fungi vector 乳酸克鲁维酵母载体酵母真菌基因敲除基因编辑载体植物细胞载体 plant cell vector 农杆菌菌株Agrobacterium tumefaciens strain 植物细胞基因敲除载体 plant cell 哺乳动物细胞载体哺乳动物细胞荧光载体荧光素酶报告基因载体哺乳动物细胞基因敲除基因编辑载体杂交系统慢病毒载体腺病毒载体逆转录病毒载体杆状病毒表达载体基因干扰 RNAi载体基因/cDNA/ORF 转座子质粒系统 transposon 金黄色葡萄球菌载体 staphylococcus aureus 假单胞菌载体噬菌体 phage 不动杆菌载体双岐杆菌载体藻类表达载体链球菌载体厌氧菌载体基因治疗载体大肠杆菌基因突变体菌株细菌荧光质粒白色念珠菌载体体外转录载体谷氨酸棒杆菌载体酿酒酵母基因突变体菌株线虫载体斑马鱼载体 Zebra fish 果蝇，昆虫载体Drosophila 鱼类细胞载体 fish cell 分支杆菌载体克雷伯菌枯草芽孢杆菌基因缺失突变株基因ORF 金黄色葡萄球菌基因敲除突变株肺炎克雷伯菌基因敲除突变株
工程菌菌株
质粒菌株资料

产品编号	产品名称
L1-26	大肠杆菌BW25141(λDE3)

产品介绍

E. coli strain BW25141(λDE3)

The E. coli BW25141strain can be used to propagate plasmids with R6K conditional replicationorigin, such as integrative base vector after the excision of its insert.

BW25141 propagates R6K origin at a copy number comparableto medium copy plasmids (~15 plasmids per cell).

Strain Designation:BW25141 Source of Strain:B.L. WannerSex:F-

Chromosomal Markers: Δ(araD-araB)567,ΔlacZ4787(::rrnB-3),Δ(phoB-phoR)580,λ^-,galU95,ΔuidA3::pir⁺,recA1,endA9(del-ins)::FRT,rph-1,Δ(rhaD-rhaB)568,hsdR514

StrainComments:

·Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araBstart codon to ~8 bp into the beginning of the araD gene

·ΔlacZ4787(::rrnB-3)--: 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacementinto the region extending from near the SacII site near theN-terminus of lacZ through the promoter.

·ΔuidA3::pir⁺-- Allows the replication of plasmids withan R6Kγ origin of replication

·recA1-- :Missense mutation, altered isoelectric point. Sequenced: G to Afor Nuc. 720 (Gly 160 Asp).

·rph-1-- isa 1 bp deletion that results in frameshift over last 15 codonsand has polar effect onpyrEleading to suboptimal pyrimidine levels on minimalmedium.(Jensen 1993 JBact.175:3401)

·Δ(araD-araB)567was formerly called Δ araBAD_AH33

·Δ(rhaD-rhaB)568was formerly called Δ rhaBAD_LD78

·endA9(del-ins)::FRTwas formerly called endA_BT333

·ΔlacZ4787(::rrnB-3)wasformerly called Δ lacZ_WJ16

·ΔlacZ4787(::rrnB-3)wasformerly called::rrnB-4

·ΔuidA3::pir⁺was formerly called uidA(Δ MluI)::pir⁺

·::rrnB-3= 4 copies of rrnB inserted

·del-ins= deletion-insertion

References:

·Datsenko,KA, BL Wanner 2000. One-step inactivation of chromosomal genes inEscherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci.U.S.A. 97(12):6640-5.

·Haldimann,A, BL Wanner 2001. Conditional-replication, integration,excision, and retrieval plasmid-host systems for genestructure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.