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Organism:
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pGPTV-DHFR
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History:
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<< ATCC<< R. I. Masterson and D. I. Becker
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Description:
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The vector carrys a promoterless beta-glucuronidase (uidA) gene which allows the construction of promoter fusions. Binary vector with plant selectable marker near the T-DNA left border and a uidA reporter gene. The marker and uidA are divergently transcribed, and can be esaily removed or replaced. The order of major features of this plasmid is: EcoRI-pAnos-SstI-uidA SmaI-XbaI-SalI-HindIII-pnos-BglII-dhfr-BamHI-pAg7-T-DNA-BglII-RK2-ori-kanR.
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Other Collection No.:
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ATCC77390
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Reporter Gene:
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uidA
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Vector Category:
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promoter cloning vectors
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Host Range:
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Agrobacterium tumefaciens Escherichia coli Higher plants
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Vector Type:
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plasmid
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Replication Origin:
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RK2 in Escherichia coli and T-DNA in Agrobacterium
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Select Marker:
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kan for Escherichia coli and Agrobacterium and dhfr for plants
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli HB101
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Size(kb):
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13.6
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