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Organism:
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pU2X1
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History:
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<< ATCC<< J. A. Bruenn
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Description:
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Description Shuttle expression vector using Ustilago maydis promoter gap (glyceraldehyde phosphste dehydrogenase) or hsp70 to drive expression of the cloned insert in the BamHI or KpnI cloning site respectively. Constructed from pUXV3 by inserting a 2.1 kb hsp70 promoter and termination cassette into the KpnI site, creating a unique KpnI cloning site between the promoter and terminator. Differs from pU2X2 only in the oriention of the hsp70 promoter-terminator cassette. The order of the major features in the plasmid is : Ustilago maydis ARS - ampR-pMB1 ori -5' lacZ fragment - EcoRI - SacI - hsp70promoter -> KpnI - hsp70 terminator -> gap promoter -> BamHI <- cbxR - 3' lacZ fragment.
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Other Collection No.:
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ATCC77466
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Vector Category:
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shuttle vector, cloning vector, expression vector
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Host Range:
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Escherichia coli/Ustilago maydis
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Vector Type:
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plasmid
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Replication Origin:
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pMB1
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Select Marker:
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cbx
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Promoter:
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gap(Ustilago maydis), hsp70
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli HB101
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Size(kb):
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8.1
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编号：54643153464444 备案号：浙ICP备11028186号-1