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酿酒酵母载体

pTRP56,ATCC37306 酿酒酵母穿梭载体

产品编号 产品名称
pTRP56,ATCC37306 酿酒酵母穿梭载体

产品介绍

Organism: pTRP56
History: << ATCC<< A. Miyajima
Description: Plasmid pTRP56 serves as the starting material for preparation of the vector primer. This vector primer can be prepared by digestion with BamHI and KpnI. The BamHI site provides the cohesive end at which the vector primer is joined to the linker fragment. The 3' protruding end of the KpnI site can be used for tailing with T residues which can form basepairs with the polyA tract of the mRNA. Close to the KpnI site the primer fragment also contains the polyadenylation signal from the TRP5 gene of S. cerevisiae. Plasmid pTRP56 has the ability to replicate in S. cerevisiae due to the presence of an element (ARS) allowing autonomous replication in S. cerevisiae. The vector contains an origin of replication for E. coli and selection markers for S. cerevisiae (TRP1) and for E. coli (amp, trpC)
Other Collection No.: ATCC37306
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Host Range: Saccharomyces cerevisiae, Escherichia coli
Vector Type: plasmid
Replication Origin: pMB1
Select Marker: amp, tet, TRP1
Shipped Host: Freeze-dried Escherichia coli MC1061
Size(kb): 6.2

基本信息

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