Organism:
|
pYAC-RC
|
History:
|
<< ATCC<< D. Marchuk
|
Description:
|
A yeast artificial chromosome vector for genomic library vonstruction using DNA digested with infrequent restriction enzymes. Constructed by removing the ClaI and SalI sites from pYAC3 and inserting a polylinker in the SnaBI site within SUP4. SUP4. Still functions. Due to ligation compatibility with sites in the polylinker, DNA fragments from BssHII, EagI, NaeI, NarI, NruI, SmaI and XhoI digests may also be cloned in this vector.
|
Other Collection No.:
|
ATCC37610
|
Growth Conditions:
|
37°C 1 day(s)
|
Biosafety Level:
|
1
|
Host Range:
|
Saccharomyces cerevisiae, Escherichia coli
|
Vector Type:
|
yeast artificial chromosome
|
Replication Origin:
|
pMB1, ARS1
|
Select Marker:
|
amp, HIS3, SUP4, TRP1, URA3
|
Shipped Host:
|
Freeze-dried Escherichia coli MC1061
|
Size(kb):
|
11.7
|