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Organism:
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pEMBLYe30
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History:
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<< ATCC<< G. Cedareni
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Description:
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Phasmid pEMBL Ye30 has properties similar to those of pEMBL Ye23. It contains the LEU2 gene from S. cerevisiae as a selsction marker for S. cerevisiae and E. coli. The vector carries unique cloning sites in the lacZalpha sequwnce for BamHI, HindIII and SmaI. Phasmid pEMBL Ye30 comprises a 5.5 kb XhoI- HindIII fragment from pCV13 containing the 2 micron yeast plasmid replicon and the LEU2 gene, inserted into the ClaI site of pEMBL9.
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Other Collection No.:
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ATCC37404
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Reporter Gene:
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lacZ'
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Vector Category:
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shuttle vector, cloning vector, YI-type(episomal) shuttle vector, vector permitting construction of fusion protein, vector permitting production of single-stranded DNA
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Host Range:
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Saccharomyces cerevisiae Escherichia coli
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Vector Type:
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phagemid
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Replication Origin:
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pMB1, f1 (E. coli) .2 micron (S. cervisiae)
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Select Marker:
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amp, URA3
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli K-12 71/18
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Size(kb):
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9.5
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Multi Clone Site:
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Yes
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