|
Organism:
|
pEMBLYe23
|
|
History:
|
<< ATCC<< G. Cedareni
|
|
Description:
|
The phasmid can be used as cloning vectors for S. cerevisiae and E. coli. It is derived from pEMBL9 by insertion of yeast sequences and can be rapidly switched in E. coli between the plasmid and phage modes of replication. It contains the ampicillin resistance gene and the lacZalpha gene for selection and detection, respectively, of recombinant plasmid in E. coli. It can be propagated in S. cerevisiae because of the presence of the 2 micron plasmid replicon, and in E. coli. The phasmid differs in their cloning sites and in its genetic marker which is used for selection of transformation in S. cerevisiae. The vector pEMBL Ye23 contains the yeast URA3 gene which can be used as a selective marker in S. cerevisiae and E. coli. Phasmid pEMBL Ye23 comprises a 3.1 kb HindIII fragment from Yep24 containing the URA3 gene and the 2 micron yeast phasmid replicon, inserted into the ClaI site of pEMBL9.
|
|
Other Collection No.:
|
ATCC37400
|
|
Growth Conditions:
|
37°C 1 day(s)
|
|
Biosafety Level:
|
1
|
|
Reporter Gene:
|
lacZ'
|
|
Vector Category:
|
shuttle vector, cloning vector, YI-type(episomal) shuttle vector, vector permitting construction of fusion protein, vector permitting production of single-stranded DNA
|
|
Host Range:
|
Saccharomyces cerevisiae Escherichia coli
|
|
Vector Type:
|
phagemid
|
|
Replication Origin:
|
pMB1, f1 (E. coli) .2 micron (S. cervisiae)
|
|
Select Marker:
|
amp, URA3
|
|
Suggested Host:
|
rec- strain of E. coli
|
|
Shipped Host:
|
Freeze-dried Escherichia coli K-12 71/18
|
|
Size(kb):
|
7.4
|
编号：54643153464444 备案号：浙ICP备11028186号-1