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Organism:
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YIp363
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History:
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<< ATCC<< C. J. Lusty
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Description:
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The EcoRI site is not unique. One of 3 promoter-cloning, YI type shuttle vectors with LEU2 selection in S. cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multipe cloning sites differering in reading frame. The sequence and reading frame of the multiple cloning sequence is : 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.
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Other Collection No.:
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ATCC37746
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Reporter Gene:
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lacZ'
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Vector Category:
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shuttle vector, cloning vector, promoter-cloning vector, YI-type(integrating) shuttle vector
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Host Range:
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Saccharomyces cerevisiae Escherichia coli
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Vector Type:
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plasmid
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Replication Origin:
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pMB1
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Select Marker:
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amp, LEU2
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli HB101
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Size(kb):
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7
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Multi Clone Site:
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Yes
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编号：54643153464444 备案号：浙ICP备11028186号-1