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Organism:
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YIp356
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History:
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<< ATCC<< C. J. Lusty
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Description:
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Plasmid Yip356 is a useful vector for studying control of gene experssion in S. cerevisiae. The vector contains the E. coli lacZ gene, encoding beta-galactosidase. The lacZ gene lacks the promrter, operator, translation-initiation codon and the first eight codons. The plasmid harbors within the lacZ gene the polylinker regioin from M13mp18. DNA fragment containing a promoter and translation-initiation region can be inserted into these sites, to form fusion proteins, if the reading frames are properly aligned. The formation of beta-galactosidase fusion proteind in S. cerevisiae can be detected on solidified midium agar plates by addition to the medium of the colorimetric substrate X-gal. The vector can replicate in E. coli but not in S. cerevisiae.
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Other Collection No.:
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ATCC37749
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Reporter Gene:
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lacZ'
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Vector Category:
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shuttle vector, cloning vector, promoter-cloning vector, YI-type shuttle vector
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Host Range:
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Saccharomyces cerevisiae Escherichia coli
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Vector Type:
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plasmid
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Replication Origin:
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pMB1
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Select Marker:
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amp, pyrF(E. coli) URA3(yeast)
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli HB101
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