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Organism:
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YIp352
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History:
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<< ATCC<< A. Tzagoloff
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Description:
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plasmid YIp352 can be used as a cloning vector for Saccharomyces cerevisiae and Escherichia coli and as a vector for selecting DNA sequences containing a replicon for S. cerevisiae. DNA sequences can be cloned in the lacZalpha region providing detection of E. coli transformants containing recombinant plasmids on the basis of their Lac- phenotype. Bacteria harboring recombinant plasmids make colorless colonies. Plasmid YIp325 contains several unique restriction sites in the lacZalpha region, downstream from the lacZ-initiation codon; the sequence with the cloning sites is the same as in M13mp18.
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Other Collection No.:
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ATCC37675
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Reporter Gene:
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lacZ'
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Vector Category:
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shuttle vector, cloning vector, integrating vector
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Host Range:
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Saccharomyces cerevisiae Escherichia coli
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Vector Type:
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plasmid
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Replication Origin:
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pMB1
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Select Marker:
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amp, pyrF(E. coli) URA3(yeast)
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Promoter:
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lac
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Shipped Host:
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Freeze-dried Escherichia coli RR1
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Size(kb):
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4.3
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Multi Clone Site:
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Yes
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