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Organism:
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pHSREM4
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History:
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<< ATCC<< D. C. Knipple
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Description:
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The KpnI/SacI fragment of pHSREM1 containing the hsp70 transcription module was ligated into KpnI/SacI digested Bluescript SK-. Major Drosophila hsp70 regulator element are:2 heat shock consensus elements(-85 to -72 and -62 to -49), a TATA box(-33 to -26) and transcription initiation site (+1) in the 5' segment; the termination triplet and polyA site in the 3' segment. One of a series of plasmid vectors for expression genes under the control of the Drosophila hsp70 promoter and terminator. The transcription module is flanked by restriction sites, T7 and T3 promoters, and primers for Sanger sequencing. There is also an M13 origin of replication. A KpnI/SacI double digest releases the hsp70 transcription module, for subsequent ligation into other plasmids sush as those containing Drosophila P element. KpnI BglII NotI XhoI-promoter-MCS-terminator-SpeI XbaI NotI EagI BstXI SacII SacI.
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Other Collection No.:
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ATCC37645
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Growth Conditions:
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37°C 1 day(s)
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Biosafety Level:
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1
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Vector Category:
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vector permitting RNA synthesis in vitro, vector permitting production of single-stranded DNA, vector containing primer sites useful for sequencing, contains easialy purifiable component for construction
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Host Range:
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Vertebrate cell Escherichia coli
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Replication Origin:
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pMB1, M13
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Select Marker:
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amp
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Promoter:
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hsp70, T3, T7
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Suggested Host:
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rec- strain of E. coli
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Shipped Host:
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Freeze-dried Escherichia coli HB101
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Size(kb):
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3.4
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Multi Clone Site:
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Yes
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