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细菌广宿主载体 bacateria broad range host vector

pGEc41 大肠杆菌载体

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pGEc41 大肠杆菌载体

产品介绍

This alkBAC/alkR system is the first P. putida expression system described to be identically controlled in Pseudomonas and E. coli. Consists of the broad host range vector pLAFR1 (21.6 kb) and a 30 kb EcoRI fragment carrying the alkR locus and part of the alkBAC operon (alkBFGH and alkST genes). The alk regulon of the OCT plasmid is encoded by two distinct regions: alkBAC and alkR. While the plasmid-borne alk system for alkane hydroxylation and alkanol dehydrogenation is active in P. putida, it is active in E. coli only in fadR mutants with constitutive fatty acid degradation enzymes. Octane vapour is used as sole source of carbon and energy. AlkR locus is strictly necessary for expression of the 7.5 kb alkBAC operon. This expression is very efficient in E. coli. The cloned alk genes are covered by U.S. patent 5,173,428.

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