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大肠杆菌宿主菌株 E.coli

大肠杆菌 INV110,甲基化酶修饰菌株

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大肠杆菌 INV110,甲基化酶修饰菌株

产品介绍

The INV110 strain is a significant improvement over the JM110 strain. The INV110 strain has been engineered to carry an endA1 mutation, which eliminates the non-specific Endonuclease I, for improved plasmid DNA preparation. Mutation in the methylation-dependent restriction system Δ(mcrC-mrr) allows transformation of genomic DNA. This strain also has the lacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. The INV110 strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids) and allows isolation of single-stranded DNA (ssDNA). In addition, the F´ episome carries the lacIq repressor for inducible expression from trctac, and lac promoters using IPTG.

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