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细菌基因敲除载体

pcrF11 枯草芽孢杆菌基因敲除载体

产品编号 产品名称
pcrF11 枯草芽孢杆菌基因敲除载体

产品介绍

Description

The plasmid is used for the expression of crRNA in B. subtilis,and can replicate in E. coli

Plasmid pcrF11 was constructed by assembling four fragments including (a) a B. subtilis replicon RepF that is compatible with the replicon RepA in plasmid pHT‐XCR6 and regions for crRNA array and homologous arms insertion, (b) a E. coli replicon ColE1, (c) a single‐strand origin of B. subtilis and a broad‐spectrum promoter Pbs that is functional in E. coli and B. subtilis, and (d) a kanamycin resistance gene used for screening in both E. coli and B. subtilis. T.

Publication Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., & Liu, L. (2020). CAMERS‐B: CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for Bacillus subtilis. Biotechnology and Bioengineering.

基本信息

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