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pADH

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pADH

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Description GAL1/10 bidirectional promoter of pESC-LEU was substituted with 725 bp of ADH1 promoter. We cut out ADH1 promoter from the pYM-N6 plasmid (Euroscarf) with EcoRV and SpeI and cloned in SmaI and SpeI of pESC-LEU. I contains LEU2 as yeast selection marker
Publication Galli A, Della Latta V, Bologna C, et al. Strategies to optimize capsid protein expression and single-stranded DNA formation of adeno-associated virus in Saccharomyces cerevisiae. Journal of applied microbiology. 2017; 123(2):414-428. [pubmed]

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