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细菌广宿主载体 bacateria broad range host vector

细菌基因敲除自杀载体pME6250B,pME6250S,pME6251H,pME6256,pME6259,pME6261,pME6284,pME6710,pME

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细菌基因敲除自杀载体pME6250B,pME6250S,pME6251H,pME6256,pME6259,pME6261,pME6284,pME6710,pME

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pME6250B pME3049 with a 1.7-kb genomic fragment of P. fluorescens CHA630 This study (Fig. (Fig.1)1)
pME6250S pME3049 with a 11.1-kb genomic fragment of P. fluorescens CHA630 This study (Fig. (Fig.1)1)
pME6251H pME3049 with a 9.8-kb genomic fragment of P. fluorescens CHA0 This study (Fig. (Fig.1)1)
pME6256 pUK21 with a 2-kb BamHI-SphI fragment of pME6250S This study
pME6259 pME6014 with a 1.1-kb BglII fragment of pME6256, containing a fragment of 0.7 kb upstream of phlA and a phlA′-′lacZ translational fusion at the BglII site in phlA This study (Fig. (Fig.1)1)
pME6261 pME6030 with a 2-kb BamHI-XhoI fragment of pME6256, containing phlA This study (Fig. (Fig.1)1)
pME6284 pBluescript II KS+ with a 2.9-kb EcoRV-SmaI fragment of pME6250S This study
pME6710 pME6016 with a 1.1-kb BglII fragment of pME6256, containing a phlA-lacZ transcriptional fusion at the BglII site in phlA This study
pME6824 pUC18 with a 2.3-kb NruI-SpeI fragment of pME6284, containing phlF This study
pME6825 pME3280a with a 2.3-kb KpnI-HindIII fragment of pME6824, containing phlF

This study (Fig. (Fig.1)1)







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